To explore the feasibility of bio-functional modification by loading RGD peptide on the surface of pure titanium. Studies have pointed out that RGD peptide functionally modified titanium sheets have good biocompatibility, and this titanium surface functionalization modification method is expected to be used to improve titanium implants and improve bone bonding.
The active layer of TiO2 nanotube-polydopamine-RGD peptide was constructed on the surface of pure titanium by means of electrochemical and molecular self-assembly technology, and the surface shape of each group of titanium sheets was subjected to field emission scanning electron microscopy (FESEM) and X-ray photoelectron spectroscopy (XPS). Appearance and elemental composition analysis. Mouse bone marrow stromal stem cells (BMSCs) were cultured, and the titanium tablets before and after treatment were evaluated for in vitro biological activity.
SEM and XPS showed that after two-step anodization, honeycomb porous TiO2 nanotubes were formed on the surface of pure titanium. Dopamine self-polymerized on the TiO2 nanotubes to form a polydopamine coating. At the same time, RGD polypeptide was coupled to the external branch, and finally in pure titanium The TiO2 nanotube-polydopamine-RGD polypeptide active layer was successfully formed on the surface. In vitro cell culture showed that the active layer was conducive to cell adhesion, proliferation and differentiation.